· In HPLC the mobile phase is a liquid whereas in Gas Chromatography the mobile phase or carrier is a gas.
· HPLC is useful for analysis of samples which are liable to decompose at higher temperatures. GC involves
high temperatures so compounds are stable at such temperatures.
· Gas Chromatography is applied for analysis of volatile compounds whereas non volatile compounds can be
easily analyzed on HPLC
· Gas Chromatography cannot be used for analysis of high molecular weight molecules whereas HPLC has
applications for separation and identification of very high molecular weight compounds
· HPLC requires higher operating pressures than GC because liquids require higher pressures than gases for
transport through the system
· HPLC columns are short and wide in comparison to GC columns